Scientific Director: Eric Svensson, MD, PhD
Technical Director: Linda Degenstein
Website: transgenic.bsd.uchicago.edu
The Transgenic Mouse and Embryonic Stem Cell Facility provides investigators with genetically manipulated mice through transgenic technology or embryonic stem (ES) cell manipulation. The facility provides a comprehensive set of technical services, has a fully operational construction and gene targeting service, and offers an annual course in Mouse Handling and Breeding.
Services
- Transgenic mouse production from founder through F1 Stage
- ES cell technology mouse production (e.g., knockouts, knockins, conditional knockouts)
- Gene design and construction using recombineering and traditional cloning
- ES cell line development and gene targeting and culturing
- Mouse embryonic fibroblast (MEF) cell production and embryo rederivation
- Various breeding services and genetically engineered mouse model line maintenance
- DNA preparation from ES cell lines for the construction of gene targeting constructs
- Maintenance of Mox 2Cre, ROSA26 Red/Green, FLPe mouse lines for the investigator’s use in breeding conditional and inducible conditional mouse models
