Scientific Director: Eric Svensson, MD, PhD
Technical Director: Linda Degenstein, AB
Website: transgenic.bsd.uchicago.edu
The Transgenic Mouse and Embryonic Stem Cell Facility provides investigators with genetically manipulated mice through transgenic technology or embryonic stem (ES) cell manipulation. The Facility provides a comprehensive set of technical services and has a fully operational construction and gene targeting service.
Services
- Transgenic mouse production from founder through F1 Stage
- ES cell technology mouse production (e.g., knockouts, knockins, conditional knockouts)
- ES cell gene targeting and culturing
- Embryo rederivation
- Mouse embryonic fibroblast (MEF) cell production
- Timed pregnancies of various strains and lines of mice
- Various breeding services and genetically engineered mouse model line maintenance
- DNA preparation from ES cell lines
- Design and construction of transgenic or ES cell targeting vectors
- PCR and Southern blot analysis of existing ES cell lines and constructs
- Training in the breeding and handling of mice, isolation of genomic DNA from mouse tissues, and genomic DNA analysis through Southern blot hybridization
