Scientific Director: Anne Sperling, PhD
Technical Director: Carol McShan, BA/BS
Website: fitchantibodies.bsd.uchicago.edu
(Read an article about this core in Pathways to Discovery.)
The Frank W. Fitch Monoclonal Antibody Facility provides a growing range of standard and custom services related to monoclonal antibodies, protein purification and conjugation, tissue culture technique, and frozen cell storage. The facility can offer advice on the development of custom murine ELISA assays and has extensive experience in the area of hamster ELISAs. The facility's collection of hybridomas and their related antibodies is continuing to expand and the cost per milligram is only a fraction of that charged by commercial sources.
Services
- Novel monoclonal antibody production
- Custom fusions for DNA analysis of hybridomas
- Subcloning of hybridoma cell lines
- Freezing and thawing of hybridoma cell lines
- ELISA screening and quantitation of mIgG
- Monoclonal antibody isotyping (rat & mouse)
- Antibody purification using protein A or G columns
- Ab conjugation to FITC or Biotin
- Pre-conjugated anti-CD3, 4 & 8 to Alexa 488 and 633
- Long-term cell line storage
- Extensive Antibody/Hybridoma Bank
- Other specialized & customized services
- Project consultation & planning
- Education and teaching monoclonal antibody production techniques
