Scientific Director: Anne Sperling, PhD
Technical Director: Ryan Duggan, BA
Website: ucflow.uchicago.edu
The Flow Cytometry Facility provides access to state-of-the-art technology and quantitative analytical approaches to measure molecular and cellular function. The facility is designed to meet the widespread needs for specialized cytologic analysis by providing instrumentation for cell sorting and bench-top analysis of subcellular components using multiparametric fluorescence quantitation technology.
Services
- Bench-top analyses ranging from simple protein expression assays using fluorescent reporters to highly complex 12-color functional assay
- Cell sorting at bulk rates of 30,000 cells per second deposited in tubes or in microtiter formats
- Fluorescence quantitation and spatial resolution combined with morphometric analysis using the ImageStream X Analyzer
- Multiplex cytokine quantitation assays using the Luminex platform
- Development of novel fluorescence-based detection assays and applications
- Data analysis
- Education and training in use and application of all instrumentation and software analysis programs within the facility
